Journal: European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery
Article Title: Adipocytokine resistin correlates with oxidative stress and myocardial injury in patients undergoing cardiac surgery.
doi: 10.1093/ejcts/ezt634
Figure Lengend Snippet: Figure 3: Correlations between increases in resistin levels and myocardial damage as measured by TnT release and oxidative stress measured by MPO release in patients (n = 32) with CAD during and after an elective on-pump CABG with CPB. Spearman’s rank correlation coefficient was used due to the non-parametrical distri- bution of the variables. (A) Resistin levels are presented as the maximal change in resistin levels from the baseline level. The greatest change in resistin levels existed between the first and the last point of measurement in nearly all of the patients. TnT was measured after the induction of anaesthesia (baseline) and 24 h after the onset of CPB with the changes being plotted. A strong positive correlation was found between resistin levels and the extent of TnT release (r = 0.489, P = 0.005**). (B) Resistin levels are presented as the change in resistin levels between baseline levels and levels measured 1 min after the removal of the ACC. MPO release is pre- sented as the maximal change from the baseline. The greatest MPO levels were reached 1 and 15 min after the removal of the ACC. A strong positive correlation was found (r = 0.563, P = 0.001***) between resistin levels and MPO release.
Article Snippet: The concentrations of the adipocytokines (adiponectin, adipsin, leptin and resistin), IL-6, 8-isoprostane and MPO in plasma samples were determined by enzyme-linked immunosorbent assay (ELISA) using the following reagents: adiponectin, adipsin, leptin and resistin: DuoSet ELISA, R&D Systems Europe, Ltd, Abindgon, UK; IL-6: PeliPair ELISA, Sanquin, Amsterdam, Netherlands; 8-isoprostane: Cayman, Ann Arbor, MI, USA; and MPO: Hycult Biotechnology, Uden, Netherlands.
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